Heavy acetylpeptide standard concentration curve in simple matrix (mitochondrial lysate)						
						
spiked peptides: LVSSVSDLPKacR (HMGCS2 protein), MVQKacSLAR (HMGCS2 protein), AFVDSCLQLHETKacR (LCAD protein), YAPVAKacDLASR (SDHA protein), LFVDKacIR (ATP5J protein), and AFGGQSLKacFGK (SdhA protein)						
						
replicate acquisitions (and runorder):						
						
simple matrix: bovine six protein mix (Michrom)						
						
	peptide spike 	replicate				
	level [in fmol]					
110914_0073_A0_rep1_R_	0	1				
110914_0007_A1_rep1_	0.0041	1				
110914_0008_A2_rep1_	0.0123	1				
110914_0009_A3_rep1_	0.037	1				
110914_0010_A4_rep1_	0.11	1				
110914_0011_A5_rep1_	0.33	1				
110914_0012_A6_rep1_	1	1				
110914_0013_A7_rep1_	3	1				
110914_0014_A8_rep1_	25	1				
						
110914_0074_A0_rep2_R_	0	2				
110914_0020_A1_rep2_	0.0041	2				
110914_0021_A2_rep2_	0.0123	2				
110914_0022_A3_rep2_	0.037	2				
110914_0023_A4_rep2_	0.11	2				
110914_0024_A5_rep2_	0.33	2				
110914_0025_A6_rep2_	1	2				
110914_0026_A7_rep2_	3	2				
110914_0027_A8_rep2_	25	2				
						
110914_0075_A0_rep3_R_	0	3				
110914_0030_A1_rep3_	0.0041	3				
110914_0031_A2_rep3_	0.0123	3				
110914_0032_A3_rep3_	0.037	3				
110914_0033_A4_rep3_	0.11	3				
110914_0034_A5_rep3_	0.33	3				
110914_0035_A6_rep3_	1	3				
110914_0036_A7_rep3_	3	3				
110914_0037_A8_rep3_	25	3				
						

Six lysine-acetylated synthetic peptides containing stable isotope labeled lysine or arginine residues (13C615N2-Lys and 13C615N4-Arg, respectively) 						
						
						
Heavy acetylpeptide standard concentration curve in compolex matrix (mitochondrial lysate)						
						
spiked peptides: LVSSVSDLPKacR (HMGCS2 protein), MVQKacSLAR (HMGCS2 protein), AFVDSCLQLHETKacR (LCAD protein), YAPVAKacDLASR (SDHA protein), LFVDKacIR (ATP5J protein), and AFGGQSLKacFGK (SdhA protein)						
						
replicate acquisitions (and runorder):						
						
complex matrix: "mitochondrial lysate from mouse liver, 0.3 ug injected on column" 						
						
	peptide spike 	replicate				
	level [in fmol]					
110914_0038_A0Mito_rep1_	0	1				
110914_0039_A9_rep1_	0.0041	1				
110914_0040_A10_rep1_	0.0123	1				
110914_0041_A11_rep1_	0.037	1				
110914_0042_A12_rep1_	0.11	1				
110914_0044_A13_rep1_	0.33	1				
110914_0045_A14_rep1_	1	1				
110914_0046_A15_rep1_	3	1				
110914_0047_A16_rep1_	25	1				
						
110914_0049_A0Mito_rep2_	0	2				
110914_0050_A9_rep2_	0.0041	2				
110914_0051_A10_rep2_	0.0123	2				
110914_0052_A11_rep2_	0.037	2				
110914_0053_A12_rep2_	0.11	2				
110914_0055_A13_rep2_	0.33	2				
110914_0056_A14_rep2_	1	2				
110914_0057_A15_rep2_	3	2				
110914_0058_A16_rep2_	25	2				
						
110914_0060_A0Mito_rep3_	0	3				
110914_0061_A9_rep3_	0.0041	3				
110914_0062_A10_rep3_	0.0123	3				
110914_0063_A11_rep3_	0.037	3				
110914_0064_A12_rep3_	0.11	3				
110914_0066_A13_rep3_	0.33	3				
110914_0067_A14_rep3_	1	3				
110914_0068_A15_rep3_	3	3				
110914_0069_A16_rep3_	25	3				
						
						
Six lysine-acetylated synthetic peptides containing stable isotope labeled lysine or arginine residues (13C615N2-Lys and 13C615N4-Arg, respectively)