QS7 - Pull Down

DTASelect v1.9.01
/net/maccoss/vol1/home/hoopmann/QS7
/net/maccoss/vol2/software/dbase/SGD/SGD-orf-040204-rand.fasta
EE-normalized ? in SQT format.
-1 0.28 -2 0.28 -3 0.28 -d 0.12 -y 1 -i 0.2 -p 2 -o -Smn 7 -e rand_
Jump to the summary table.

sequest.params modifications:

*	X	0.0
#	X	0.0
@	X	0.0
Static	C	57.0

true	Use criteria
0.28	Minimum +1 XCorr
0.28	Minimum +2 XCorr
0.28	Minimum +3 XCorr
0.12	Minimum DeltCN
1	Minimum charge state
3	Maximum charge state
0.2	Minimum ion proportion
1000	Maximum Sp rank
-1.0	Minimum Sp score
Include	Modified peptide inclusion
Half	Tryptic status requirement
true	Multiple, ambiguous IDs allowed
Ignore	Peptide validation handling
	Sequences must include all of
	Sequences must include pattern
	Sequences can contain none of
	Preceding residue must be one of
	C terminal residue must be one of
7	Minimum sequence length
100	Maximum sequence length
0	Sequence completeness required
XCorr	Purge duplicate peptides by protein
false	Include only loci with unique peptide
true	Remove subset proteins
Ignore	Locus validation handling
rand_	Exclude protein names matching
0	Minimum modified peptides per locus
10	Minimum redundancy for low coverage loci
2	Minimum peptides per locus

Locus Key:

Validation Status

Locus

Sequence Count

Spectrum Count

Sequence Coverage

Length

MolWt

Descriptive Name

Similarity Key:

Locus

# of identical peptides

# of differing peptides

YDR382W

52.7%

110

11050

4.1

RPP2B SGDID:S0002790, Chr IV from 1239480-1239812, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.07037.07037.2	0.6849	0.4674	1640.21	1638.905	1	1331.1	71.9%	1	Y.LLLVQGGNAAPSAADIK.A	2
*	032305-alex-pull-down-02.07809.07809.2	0.5973	0.265	1202.89	1203.378	2	1472.1	85.0%	1	R.INELLSSLEGK.G	2
*	032305-alex-pull-down-02.06518.06518.3	0.7637	0.5537	2563.1	2564.682	1	3089.5	44.8%	1	K.FATVPTGGASSAAAGAAGAAAGGDAAEEEK.E	3

YLR212C

249

46.7%

473

52628

4.7

TUB4 SGDID:S0004202, Chr XII from 566283-564862, reverse complement, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.00987.00987.3	0.5999	0.232	2012.54	2014.113	16	490.4	37.5%	2	K.EHAIGTDGLSQLPDSSTER.D	3
*	032305-alex-pull-down-02.00296.00296.2	0.3715	0.2131	1141.75	1141.225	96	268.9	62.5%	1	R.DDDTKPFFR.E	2
*	032305-alex-pull-down-02.15862.15862.3	0.8326	0.5492	2123.51	2126.404	1	3113.1	52.8%	4	R.AIMMDSEPSVIADVENTFR.G	3
*	032305-alex-pull-down-02.15854.15854.2	0.6593	0.5123	2125.89	2126.404	1	1469.4	75.0%	5	R.AIMMDSEPSVIADVENTFR.G	2
*	032305-alex-pull-down-02.11864.11864.2	0.3395	0.4084	1765.55	1765.874	1	2345.3	82.1%	2	K.EIDSTDNFEGFQLLH.S	2
*	032305-alex-pull-down-02.19446.19446.3	0.3849	0.5442	3779.84	3782.035	1	1153.2	26.4%	1	K.EIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDR.Y	3
*	032305-alex-pull-down-02.11366.11366.2	0.7161	0.4353	1779.95	1780.031	1	3084.2	83.3%	27	R.SSEVVVQSYNTILALR.R	2
*	032305-alex-pull-down-02.11330.11330.3	0.5567	0.4302	1780.04	1780.031	1	1634.9	50.0%	3	R.SSEVVVQSYNTILALR.R	3
*	032305-alex-pull-down-02.05457.05457.2	0.3212	0.138	1051.11	1051.231	5	750.7	75.0%	3	Q.SYNTILALR.R	2
*	032305-alex-pull-down-02.12179.12179.3	0.3003	0.1701	2480.63	2478.762	1	983.8	35.2%	1	R.RLIEDSDATVVFDNASLLNISGK.V	3
*	032305-alex-pull-down-02.14132.14132.2	0.6203	0.3599	2321.23	2322.574	1	2078.3	66.7%	80	R.LIEDSDATVVFDNASLLNISGK.V	2
*	032305-alex-pull-down-02.14234.14234.3	0.709	0.452	2322.05	2322.574	1	2447.2	45.2%	95	R.LIEDSDATVVFDNASLLNISGK.V	3
*	032305-alex-pull-down-02.24544.24544.3	0.6164	0.4887	4181.9	4179.644	1	1835.8	30.6%	2	R.SPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDK.V	3
*	032305-alex-pull-down-02.21890.21890.3	0.4688	0.1818	3221.06	3223.501	1	1271.7	32.4%	1	A.KGAFLNNYNVGDLFQSMQNVQDEFAESR.E	3
*	032305-alex-pull-down-02.19946.19946.3	0.7526	0.5173	3094.07	3095.326	1	4789.7	46.2%	14	K.GAFLNNYNVGDLFQSMQNVQDEFAESR.E	3
*	032305-alex-pull-down-02.17498.17498.3	0.3812	0.4753	2818.73	2820.019	1	2293.9	44.6%	2	F.LNNYNVGDLFQSMQNVQDEFAESR.E	3
*	032305-alex-pull-down-02.15486.15486.3	0.2901	0.4083	2314.94	2315.476	1	1401.3	34.2%	1	Y.NVGDLFQSMQNVQDEFAESR.E	3
*	032305-alex-pull-down-02.15491.15491.2	0.3273	0.4253	2315.05	2315.476	1	938.7	52.6%	1	Y.NVGDLFQSMQNVQDEFAESR.E	2
*	032305-alex-pull-down-02.19964.19964.2	0.3123	0.416	2100.89	2102.24	1	546.9	41.2%	3	V.GDLFQSMQNVQDEFAESR.E	2
*	032305-alex-pull-down-02.21297.21297.3	0.4673	0.4558	3763.82	3765.903	1	260.6	23.5%	1	Y.VAAEQDSYLDDVLVDDENMVGELEEDLDADGDHK.L	3

YHR172W

219

38.4%

823

96825

6.4

SPC97 SGDID:S0001215, Chr VIII from 448333-450804, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.02991.02991.2	0.652	0.2292	1131.55	1133.333	1	1113.9	88.9%	6	R.YTNNIPLLGK.L	2
*	032305-alex-pull-down-02.22784.22784.2	0.6209	0.1345	970.47	970.197	5	835.0	81.2%	1	K.DLLNVLIGL.E	2
*	032305-alex-pull-down-02.22167.22167.2	0.4549	0.2343	1155.39	1156.365	1	881.1	75.0%	1	K.DLLNVLIGLEG.T	2
*	032305-alex-pull-down-02.22269.22269.2	0.6416	0.2586	1257.13	1257.47	1	1503.1	81.8%	1	K.DLLNVLIGLEGT.Y	2
*	032305-alex-pull-down-02.23999.23999.2	0.5844	0.3262	1421.11	1420.646	1	1990.7	87.5%	2	K.DLLNVLIGLEGTY.I	2
*	032305-alex-pull-down-02.21980.21980.2	0.8722	0.4119	1689.15	1689.993	1	1603.8	71.4%	32	K.DLLNVLIGLEGTYIR.Y	2
*	032305-alex-pull-down-02.22052.22052.3	0.6445	0.3208	1689.83	1689.993	1	1543.3	53.6%	19	K.DLLNVLIGLEGTYIR.Y	3
*	032305-alex-pull-down-02.09167.09167.2	0.7442	0.4323	2090.13	2092.223	1	1175.1	75.0%	4	R.YFNDYEPSDPETPIEFK.I	2
*	032305-alex-pull-down-02.06521.06521.3	0.7312	0.2375	1339.04	1337.521	4	750.5	55.0%	1	R.DFNKVPNFSIR.E	3
*	032305-alex-pull-down-02.00593.00593.2	0.3684	0.1449	833.09	832.978	7	315.9	83.3%	1	K.VPNFSIR.E	2
*	032305-alex-pull-down-02.06854.06854.2	0.7774	0.2756	1558.35	1559.714	1	2173.3	87.5%	4	R.ELEQIINETEVNK.Q	2
*	032305-alex-pull-down-02.21398.21398.2	0.3984	0.4569	1860.37	1862.151	1	1540.4	76.9%	1	K.QMELLYNIYEEIFR.E	2
*	032305-alex-pull-down-02.21406.21406.3	0.5507	0.239	1861.52	1862.151	1	1182.5	48.1%	1	K.QMELLYNIYEEIFR.E	3
*	032305-alex-pull-down-02.07421.07421.2	0.551	0.5282	2178.73	2180.328	1	1691.1	73.5%	1	R.RTNQSSQEDFNNFMDSMK.N	2
*	032305-alex-pull-down-02.07389.07389.3	0.5387	0.365	2180.72	2180.328	1	1614.8	44.1%	1	R.RTNQSSQEDFNNFMDSMK.N	3
*	032305-alex-pull-down-02.08980.08980.2	0.497	0.4635	2023.71	2024.141	1	1855.6	71.9%	2	R.TNQSSQEDFNNFMDSMK.N	2
*	032305-alex-pull-down-02.10004.10004.2	0.6941	0.3823	1580.87	1581.91	1	1562.8	80.8%	6	R.LMVAFDTTVYPVPK.G	2
*	032305-alex-pull-down-02.00526.00526.2	0.7044	0.2111	981.19	982.038	2	897.6	92.9%	1	K.TDNIFDTR.D	2
*	032305-alex-pull-down-02.00843.00843.2	0.6062	0.3833	1612.69	1613.684	1	969.6	79.2%	1	R.DCDSEEDKNLLFK.M	2
*	032305-alex-pull-down-02.00872.00872.3	0.7158	0.1769	1613.69	1613.684	1	465.0	47.9%	1	R.DCDSEEDKNLLFK.M	3
*	032305-alex-pull-down-02.23678.23678.3	0.6623	0.4762	4181.24	4183.628	1	702.9	26.4%	4	R.ASLQIPTIPSNSSDITIQEINDFADLMEGSNLELYVDK.C	3
*	032305-alex-pull-down-02.05786.05786.2	0.7844	0.2299	1161.57	1162.331	1	1239.6	93.8%	7	K.LLQNFELER.Q	2
*	032305-alex-pull-down-02.00015.00015.2	0.4718	0.3516	1202.75	1203.315	1	914.2	94.4%	1	R.QSENPNNLMR.Q	2
*	032305-alex-pull-down-02.10203.10203.1	0.3489	0.3069	1394.26	1395.613	1	517.5	72.7%	1	K.NMNAILFDELSK.E	1
*	032305-alex-pull-down-02.10262.10262.2	0.6779	0.2822	1398.13	1395.613	1	1687.8	86.4%	4	K.NMNAILFDELSK.E	2
*	032305-alex-pull-down-02.00556.00556.2	0.525	0.337	1650.87	1651.816	1	1180.9	67.9%	1	R.TGAYHGSNLELYTPK.S	2
*	032305-alex-pull-down-02.00534.00534.3	0.5433	0.3591	1651.88	1651.816	1	1367.9	46.4%	1	R.TGAYHGSNLELYTPK.S	3
*	032305-alex-pull-down-02.19706.19706.2	0.6933	0.2866	1789.23	1789.128	1	1166.5	85.7%	15	K.FDINIPYPLNIIISR.T	2
*	032305-alex-pull-down-02.19828.19828.3	0.8548	0.2439	1789.82	1789.128	1	1764.3	58.9%	8	K.FDINIPYPLNIIISR.T	3
*	032305-alex-pull-down-02.04605.04605.2	0.3981	0.1726	1063.77	1064.229	6	819.6	85.7%	4	R.YQLVLQYH.S	2
*	032305-alex-pull-down-02.09796.09796.2	0.3574	0.146	1615.75	1615.841	1	804.4	75.0%	1	K.TIMEYFNQNVIDK.E	2
*	032305-alex-pull-down-02.19432.19432.2	0.9579	0.4922	2468.57	2470.803	1	2690.1	68.2%	11	R.NPTLAVAIQNELEGGLTNIMTNR.C	2
*	032305-alex-pull-down-02.19646.19646.3	0.7461	0.4498	2470.28	2470.803	1	3331.5	50.0%	61	R.NPTLAVAIQNELEGGLTNIMTNR.C	3
*	032305-alex-pull-down-02.06428.06428.2	0.7331	0.3587	1377.29	1378.574	1	1322.7	85.0%	3	K.LCQLDPVLYEK.H	2
*	032305-alex-pull-down-02.09016.09016.2	0.4938	0.3431	1584.89	1583.745	1	1760.7	83.3%	2	K.CLINFTQELSTEK.F	2
*	032305-alex-pull-down-02.08944.08944.2	0.6777	0.4629	1777.15	1779.857	1	2905.6	86.7%	7	K.FDFYDSSSVDAAGIER.V	2
*	032305-alex-pull-down-02.08932.08932.3	0.349	0.2109	1779.68	1779.857	1	1126.2	45.0%	1	K.FDFYDSSSVDAAGIER.V	3

YLL024C

36.0%

639

69470

5.1

SSA2 SGDID:S0003947, Chr XII from 97484-95565, reverse complement, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.06482.06482.2	0.823	0.4227	1457.85	1458.568	1	645.6	79.2%	2	R.TTPSFVGFTDTER.L	2
*	032305-alex-pull-down-02.01882.01882.2	0.4951	0.3647	1591.99	1592.767	1	1383.1	75.0%	1	K.NQAAMNPANTVFDAK.R	2
	032305-alex-pull-down-02.07420.07420.2	0.4008	0.4564	1728.87	1730.014	1	1299.2	78.6%	1	K.LIDVDGKPQIQVEFK.G	2 2
	032305-alex-pull-down-02.07492.07492.3	0.3679	0.1509	1728.92	1730.014	320	166.7	28.6%	1	K.LIDVDGKPQIQVEFK.G	3 3
	032305-alex-pull-down-02.11765.11765.2	0.4412	0.2165	1551.75	1551.798	1	763.5	73.1%	2	K.NFTPEQISSMVLGK.M	2 2
	032305-alex-pull-down-02.08292.08292.2	0.4378	0.3825	1895.33	1896.067	1	378.8	50.0%	1	K.VNDAVVTVPAYFNDSQR.Q	2 2 2
	032305-alex-pull-down-02.08325.08325.3	0.395	0.311	1896.53	1896.067	1	818.4	39.1%	1	K.VNDAVVTVPAYFNDSQR.Q	3 3 3
	032305-alex-pull-down-02.08394.08394.2	0.7582	0.2793	1197.93	1200.381	1	1308.0	86.4%	1	K.DAGTIAGLNVLR.I	2 2 2
	032305-alex-pull-down-02.08786.08786.2	0.6877	0.4705	1659.45	1660.908	1	1888.0	86.7%	2	R.IINEPTAAAIAYGLDK.K	2 2 2
	032305-alex-pull-down-02.01929.01929.3	0.3895	0.2431	1275.98	1275.493	2	287.6	44.4%	1	R.LVNHFIQEFK.R	3 3
	032305-alex-pull-down-02.15448.15448.2	0.5092	0.5896	2577.23	2578.792	1	2484.4	56.0%	1	R.SINPDEAVAYGAAVQAAILTGDESSK.T	2 2
	032305-alex-pull-down-02.15453.15453.3	0.5349	0.5492	2578.28	2578.792	1	1534.5	38.0%	2	R.SINPDEAVAYGAAVQAAILTGDESSK.T	3 3
	032305-alex-pull-down-02.20296.20296.2	0.4588	0.4313	2556.05	2557.018	1	924.8	52.1%	2	K.TQDLLLLDVAPLSLGIETAGGVMTK.L	2 2
	032305-alex-pull-down-02.20294.20294.3	0.7227	0.5643	2557.16	2557.018	1	1645.0	39.6%	3	K.TQDLLLLDVAPLSLGIETAGGVMTK.L	3 3
	032305-alex-pull-down-02.12988.12988.3	0.3405	0.4232	2712.71	2714.992	1	1277.7	33.7%	1	K.KSEVFSTYADNQPGVLIQVFEGER.A	3
	032305-alex-pull-down-02.15656.15656.3	0.5247	0.31	2586.26	2586.817	1	2477.9	43.2%	1	K.SEVFSTYADNQPGVLIQVFEGER.A	3
	032305-alex-pull-down-02.07137.07137.2	0.4912	0.279	1185.63	1184.381	9	552.2	70.0%	5	K.FELSGIPPAPR.G	2 2 2
*	032305-alex-pull-down-02.02337.02337.3	0.4049	0.2198	2133.23	2134.305	1	864.4	42.1%	2	K.NTISEAGDKLEQADKDAVTK.K	3
*	032305-alex-pull-down-02.01632.01632.2	0.4292	0.126	1358.59	1359.581	4	529.3	63.6%	1	K.ELQEVANPIMSK.L	2

Similarities: YAL005C(13:6)
YJL034W(1:18)
YBL075C(4:15)

YGR192C

35.5%

332

35747

7.0

TDH3 SGDID:S0003424, Chr VII from 883813-882815, reverse complement, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.16104.16104.3	0.3711	0.4062	3174.68	3173.654	1	1820.1	34.3%	1	R.IALSRPNVEVVALNDPFITNDYAAYMFK.Y	3
*	032305-alex-pull-down-02.08519.08519.2	0.2834	0.4001	1611.21	1610.761	1	998.9	63.3%	1	W.GSSNVDIAIDSTGVFK.E	2
	032305-alex-pull-down-02.10224.10224.2	0.4774	0.5595	2209.43	2208.593	1	889.3	60.0%	1	K.VVITAPSSTAPMFVMGVNEEK.Y	2
	032305-alex-pull-down-02.10193.10193.3	0.3993	0.411	2209.94	2208.593	1	1175.3	36.2%	1	K.VVITAPSSTAPMFVMGVNEEK.Y	3
*	032305-alex-pull-down-02.17162.17162.3	0.4466	0.3844	2576.3	2576.924	1	985.2	37.0%	2	K.VINDAFGIEEGLMTTVHSLTATQK.T	3
	032305-alex-pull-down-02.00083.00083.2	0.5184	0.3147	1374.53	1375.522	1	1007.4	78.6%	1	R.TASGNIIPSSTGAAK.A	2
	032305-alex-pull-down-02.09674.09674.2	0.5764	0.4671	1471.43	1471.734	1	2037.2	84.6%	1	R.VPTVDVSVVDLTVK.L	2

gi|2136983|pir||I46732

34.4%

180

20576

7.9

(CONTAMINANT) Ig gamma heavy chain constant region - rabbit (fragment)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
	032305-alex-pull-down-02.00087.00087.1	0.4214	0.1755	838.3	839.023	1	347.2	78.6%	1	K.ALPAPIEK.T	1 1 1
	032305-alex-pull-down-02.00026.00026.2	0.6557	0.4052	920.27	921.102	10	522.9	78.6%	1	K.VYTMGPPR.E	2 2 2
	032305-alex-pull-down-02.16382.16382.2	0.6378	0.4622	2120.75	2120.345	1	1381.0	63.9%	3	R.SVSLTCMINGFYPSDISVE.W	2 2 2
	032305-alex-pull-down-02.18626.18626.3	0.3618	0.2481	2121.2	2120.345	10	531.6	34.7%	1	R.SVSLTCMINGFYPSDISVE.W	3 3 3
	032305-alex-pull-down-02.13472.13472.2	0.654	0.4136	1650.05	1649.794	1	1075.5	71.4%	5	K.TTPAVLDSDGSYFLY.S	2 2
*	032305-alex-pull-down-02.09452.09452.3	0.6893	0.5036	1864.67	1865.047	1	2256.2	53.1%	1	K.TTPAVLDSDGSYFLYSK.L	3
*	032305-alex-pull-down-02.09416.09416.2	0.652	0.469	1865.15	1865.047	1	1639.2	75.0%	5	K.TTPAVLDSDGSYFLYSK.L	2
	032305-alex-pull-down-02.01586.01586.2	0.5465	0.3204	1203.81	1203.34	1	378.8	77.8%	2	K.LSVPTSEWQR.G	2 2

Similarities: gi|165128|gb|AAA64252.1|(6:2)
gi|165112|gb|AAA31289.1|(4:4)

YGL030W

34.3%

105

11415

9.8

RPL30 SGDID:S0002998, Chr VII from 439094-439096,439327-439641, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.07118.07118.2	0.5583	0.4277	1816.51	1817.996	1	2454.5	81.2%	1	K.VYYFQGGNNELGTAVGK.L	2
*	032305-alex-pull-down-02.20972.20972.2	0.622	0.5337	1873.85	1874.139	1	793.8	63.9%	1	R.VGVVSILEAGDSDILTTLA.-	2
*	032305-alex-pull-down-02.20990.20990.3	0.5331	0.3945	1874.06	1874.139	1	1133.8	44.4%	1	R.VGVVSILEAGDSDILTTLA.-	3

YJR070C

10.5%

325

36165

4.9

LIA1 SGDID:S0003831, Chr X from 570512-569535, reverse complement, Verified ORF

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	032305-alex-pull-down-02.08848.08848.2	0.3655	0.3818	1500.05	1499.66	1	1689.6	79.2%	1	K.AIEYIAESFVNDK.S	2
*	032305-alex-pull-down-02.06693.06693.3	0.3787	0.283	2183.06	2183.337	1	1026.8	38.8%	1	R.HEAAEALGALGDKDSLDDLNK.A	3

/net/maccoss/vol1/home/hoopmann/QS7